Many types of antioxidant supplements are available in the private market in Japan. However, it is difficult to know which type and how much to take, as it is possible to take too many of some vitamins. Since it is difficult for general consumers to make a choice, it is important to provide information based on scientific evidence. This study investigated the effects of continuous administration of a blended supplement, Twendee X (TwX) to aging mice. When 18-month-old C57BL/6 mice were given TwX for 1 month, behavioral tests showed that special cognition and short-term memory significantly improved compared to the age-matched controls. There were no significant differences in secreted neurotrophic factors, such as nerve growth factor and brain-derived neurotrophic factor in the brain. In treadmill durability tests before and after administration, the rate of increase in running distance after administration was significantly higher than that of the untreated group. These results suggest continuous intake of TwX may improve cognition and suppress age-related muscle decline. There is no problem with overdosing, so we think it's a good idea to take the blended supplement continuously.

Free radicals continue to be produced in our body. Free radicals attack lipids and produce lipid hydroperoxide. Aggregated lipid peroxides are known to be a risk factor for developing various diseases such as arteriosclerosis and cancer. Antioxidant enzymes such as SOD and CAT that exist in the body are not sufficient to prevent these problems. Therefore, we consume antioxidants such as vitamins. Therefore, in this study, we reexamined the antioxidant activity of Twendee X, which is commonly sold as a multi-supplement [1]. In this experiment, electron spin resonance (ESR) was used to measure antioxidant activity. ESR is the only measurement method that can directly measure radicals. The results showed that Twendee X has very strong antioxidant activity. The ingredients contained in Twendee X are mainly water-soluble vitamins and amino acids. Despite this, it is surprising that Twendee X has the ability to scavenge hydroxyl radicals and superoxide radicals. This time, we will present a comparison of its antioxidant effect with other antioxidants. As future research progresses, we may be able to discover new combinations of vitamins and amino acids that strongly scavenge many types of radicals. Furthermore, if the relationship between individual radicals and disease and fatigue is clarified, it may become possible to create custom-made supplements.