ORALS

SESSION: OxidativeTueAM-R1	Ozawa International Symposium (3rd Intl. Symp. on Oxidative Stress for Sustainable Development of Human Beings)
Tue. 28 Nov. 2023 / Room: Dreams 1
Session Chairs: Haruhiko Inufusa; Koji Fukui; Session Monitor: TBA

11:35: [OxidativeTueAM01] OS Plenary
WHY TWENDEE X SUCCESSFULLY PREVENTED DEMENTIA?
Haruhiko Inufusa¹ ; Fuhua Yang¹ ; Yoshiaki Harakawa¹ ; Toshikazu Yoshikawa² ; ¹Gifu University, Gifu, Japan; ²Louis Pasteur Medical Research Center, Kyoto, Japan;
Paper Id: 152 [Abstract]

Many different types of dementia exist, with Alzheimer's disease (AD) accounting for the majority of cases. The greatest risk factor for AD is aging, which is the result of oxidative stress that has accumulated over the years. In addition, amyloid-β accumulated in the AD brain generates free radicals that cause inflammation in the surrounding neurons, leading to mitochondrial dysfunction and eventual neurodegeneration. In other words, the development of AD is the result of a vicious cycle of oxidative stress, inflammation, and mitochondrial dysfunction established over a long period of time, and the progression of AD is no exception. The pathogenesis of AD is said to occur 20 years before clinical symptoms appear. Therefore, starting treatment after the onset of symptoms could be too late to be effective. Twendee X (TwX, Oxicut®︎) is an antioxidant formula shown to have a preventive effect for human mild cognitive impairment (MCI) in a multicenter, randomized, double-blind, placebo-controlled, prospective intervention trial. Previous studies have shown that in diabetes, which hastens cognitive decline, TwX intake not only lowers blood glucose levels but also suppresses autophagy dysfunction. TwX has also been shown to positively affect neurogenesis in the hippocampal dentate gyrus, telomere elongation, and mitochondrial function. This suggests that TwX may prevent or halt the progression of dementia from all angles. This study suggests that TwX may not only prevent dementia, but may also aid in its treatment.

References:
1. Tadokoro K, Morihara R, Abe K, et al. Clinical Benefits of Antioxidative Supplement Twendee X for Mild Cognitive Impairment: A Multicenter, Randomized, Double-Blind, and Placebo-Controlled Prospective Interventional Study. J Alzheimers Dis. 2019; 71(3): 1063-1069. 2. Inufusa H. Characterization of cell protection effects of Twendee X by oxidative stress. J World Mitochondria Soc. 2016; 2: 42. 3. Haruhiko I. Composition for protection against cytotoxic effects. Tima Foundation. Patent No. 5777821. 2015-9-9. 4. Feng T, Yamashita T, Abe K, et al. In Vitro Free Radical Scavenging Activities of Dietary Supplements by Electron Spin Resonance. Brain Supplements. 2020; 2: 1-12.

SESSION: OxidativeTuePM1-R1	Ozawa International Symposium (3rd Intl. Symp. on Oxidative Stress for Sustainable Development of Human Beings)
Tue. 28 Nov. 2023 / Room: Dreams 1
Session Chairs: Christian Andre Amatore; Koji Fukui; Session Monitor: TBA

15:20: [OxidativeTuePM108] OS
OLD AGE ODOR AND OXIDATIVE STRESS
Haruhiko Inufusa¹ ; Fuhua Yang¹ ; Yoshiaki Harakawa¹ ; Toshikazu Yoshikawa² ; ¹Gifu University, Gifu, Japan; ²Louis Pasteur Medical Research Center, Kyoto, Japan;
Paper Id: 151 [Abstract]

Why does baby smell good while the elderly has a distinctive odor? According to a publication by Shiseido Research Center in 2001(1), 2-nonenal is an unsaturated aldehyde with unpleasant odor caused by oxidative degradation reactions of ω-7 unsaturated fatty acids such as palmitoleic acid and bacenic acid found on the skin surface. This means that age-related oxidative stress is a cause of 2-nonenal. In other words, an increase in oxidative stress due to aging is the cause of age-related odor. We measured the change in age-related odor by reducing oxidative stress. Antioxidant composition Twendee X (TwX) was given who has age related odor for 4 weeks. The portable odor sensor KUNKUN BODY (Konica Minolta) was used for 10 participants to measure aging odor. Seventy-four participants were also monitored questionnaires. Several participants whose odor KUNKUN BODY scores were 80-100, which dropped scores to zero after 3 weeks. Seventy-two of the 74 cases showed a decrease in age-related odor. Oxidative stress increases with age and is a cause of many diseases. The development of age-related odors suggests increased oxidative stress. Reduction of age-related odors with the antioxidant composition TwX is suggested not only to reduce bad odors but also to maintain health.

References:
1.Inufusa H. Characterization of cell protection effects of Twendee X by oxidative stress. J World Mitochondria Soc. 2016; 2: 42. 2. Haruhiko I. Composition for protection against cytotoxic effects. Tima Foundation. Patent No. 5777821. 2015-9-9. 3. Haze S, Gozu Y, Nakamura S, Kohno Y, Sawano K, Ohta H, Yamazaki K. 2-Nonenal newly found in human body odor tends to increase with aging. J Invest Dermatol. 2001 Apr;116(4):520-4.

SESSION: OxidativeTuePM2-R1	Ozawa International Symposium (3rd Intl. Symp. on Oxidative Stress for Sustainable Development of Human Beings)
Tue. 28 Nov. 2023 / Room: Dreams 1
Session Chairs: Fuhua Yang; Christian Andre Amatore; Session Monitor: TBA

16:50: [OxidativeTuePM211] OL
ALLERGIC DISEASES AND OXIDATIVE STRESS
Yoshiaki Harakawa¹ ; Fuhua Yang¹ ; Toshikazu Yoshikawa² ; Haruhiko Inufusa¹ ; ¹Gifu University, Gifu, Japan; ²Louis Pasteur Medical Research Center, Kyoto, Japan;
Paper Id: 104 [Abstract]

There are various allergic diseases existing. In Japan, about 1 in 2 of the population suffers from some sort of allergic disease, making it a national disease. Familiar examples include asthma, rhinitis, atopic dermatitis, and hay fever. These symptoms are largely related to oxidative stress, which increases due to inflammation caused by allergens such as pollen and dust invading the body. As the number of patients increases and the burden of medical costs rises, a treatment without side effects is needed. Twendee X (TwX) is a safety-tested, mixed antioxidant supplement composed of vitamins and amino acids. To evaluate whether TwX improves these symptoms, a questionnaire survey was conducted on the symptoms of several allergic diseases. The results showed that about 80% of the participants showed improvement in most diseases after taking TwX compared to before. The results suggest TwX can contribute to the future treatment of allergies as well as to the reduction of health care costs.

References:
1) Inufusa H, Greiffenclau M, Novel compositions and uses thereof. Patent Cooperation Treaty (PCT) 2,008,071,790. priority date 2006-12-15. 2) Inufusa, H. Characterization of cell protection effects of Twendee X by oxidative stress. J World Mitochondria Soc. 2016, 2, 42. 3) Inufusa, H. Composition for protection against cytotoxic effects. TIMA Foundation. Patent No. 5777821, 2015-9-9.

SESSION: OxidativeTuePM3-R1	Ozawa International Symposium (3rd Intl. Symp. on Oxidative Stress for Sustainable Development of Human Beings)
Tue. 28 Nov. 2023 / Room: Dreams 1
Session Chairs: Haruhiko Inufusa; Session Monitor: TBA

17:55: [OxidativeTuePM313] OL
ANTIOXIDANTS AS A POTENTIAL TREATMENT FOR ULCERATIVE COLITIS
Yoshiaki Harakawa¹ ; Fuhua Yang¹ ; Toshikazu Yoshikawa² ; Haruhiko Inufusa¹ ; ¹Gifu University, Gifu, Japan; ²Louis Pasteur Medical Research Center, Kyoto, Japan;
Paper Id: 105 [Abstract]

Ulcerative colitis (UC) is defined as a diffuse, nonspecific inflammation of unknown origin that continuously damages the colonic mucosa from the rectal side, often leading to erosions and ulcers. It is associated with frequent abdominal pain and sometimes diarrhea with bloody stools. There are more than 160,000 cases of the disease in Japan, but no cure has yet been established.
Although the exact cause of the disease has not yet been clarified, oxidative stress has long been implicated, as it has been observed that reactive oxygen species (ROS) increase in inflamed tissues. Reduction of ROS and improvement of oxidative stress may improve UC.
Twendee X (TwX, Oxicut®︎) is an antioxidant supplement containing eight active ingredients consisting of vitamins and amino acids; Twendee Mtcontrol (TwM, Mtcontrol®︎) is a supplement with seven active ingredients added to TwX to increase its antioxidant capacity. The therapeutic effects of antioxidants were tested in a mouse model of UC with dextran sodium sulfate (DSS), a commonly used treatment in UC research. TwX and TwM were found to ameliorate the negative physical effects of UC in the DSS model.
The results suggest that antioxidant treatment may be effective for UC.

References:
1. Japan Intractable Diseases Information Center. 2023. https://www.nanbyou.or.jp/. Accessed 15 Jun 2023.
2. Lih-Brody L, Powell SR, Collier KP, Reddy GM, Cerchia R, Kahn E, Weissman GS, Katz S, Floyd RA, McKinley MJ, Fisher SE, Mullin GE. Increased oxidative stress and decreased antioxidant defenses in mucosa of inflammatory bowel disease. Dig Dis Sci. 1996 Oct;41(10):2078-86.
3. Nakase H, Uchino M, Shinzaki S, Matsuura M, Matsuoka K, Kobayashi T, Saruta M, Hirai F, Hata K, Hiraoka S, Esaki M, Sugimoto K, Fuji T, Watanabe K, Nakamura S, Inoue N, Itoh T, Naganuma M, Hisamatsu T, Watanabe M, Miwa H, Enomoto N, Shimosegawa T, Koike K. Evidence-based clinical practice guidelines for inflammatory bowel disease 2020. J Gastroenterol. 2021 Jun;56(6):489-526.
4. Kim JJ, Shajib MS, Manocha MM, Khan WI. Investigating intestinal inflammation in DSS-induced model of IBD. J Vis Exp. 2012 Feb 1;(60):3678.