ORALS

SESSION: OxidativeTuePM2-R1	Ozawa International Symposium (3rd Intl. Symp. on Oxidative Stress for Sustainable Development of Human Beings)
Tue. 28 Nov. 2023 / Room: Dreams 1
Session Chairs: Fuhua Yang; Christian Andre Amatore; Session Monitor: TBA

16:25: [OxidativeTuePM210] OL Plenary
EFFECTS OF ANTI-OXIDANT, TWENDEE X, FOR REGENERATION OF THE VOCAL FOLD AFTER SURGERY
Shigeru Hirano¹ ; Mami Kaneko² ; Yoichiro Sugiyama³ ; Haruhiko Inufusa⁴ ; ¹Kyoto Prefectural U. of Medicine, Kyoto, Japan; ²Kyoto Prefectural University of Medicine, Kyoto, Japan; ³Department of Otolarynogolgy-Head and Neck Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan; ⁴Gifu University, Gifu, Japan;
Paper Id: 121 [Abstract]

The vocal fold is a vibratory mucosa essential for creating voice, but it is fragile after injury, and once it is scarred, voice becomes hoarse and harsh, which is difficult to recover. Regeneration of the mucosa after injury is important to avoid such dysphonia. Our previous research indicated that oxidative stress is one of the leading issues that causes scarring of the vocal fold, and anti-oxidant can preserve the tissue. The current clinical trial examined the effects of the strong anti-oxidant, Twendee X (TWX), on regeneration of the vocal fold after surgery. Materials and Methods: Ten patients received surgery for vocal fold pathologies including polyp, leukoplakia, CIS, etc. They were randomly assigned to TWX group which were treated with TWX perioperatively and control group without TWX treatment. The vocal fold function after the surgery was assessed at 1 to 3 months using stroboscopy and acoustic parameters. TWX group indicated significantly better vibratory parameters on stroboscopy at 1 month through 3 months as compared to the control group. TWX group also showed better GRBAS scale and loudness of the voice. TWX was shown to be effective for better recovery of the vocal fold after surgery with less scarring.

SESSION: OxidativeTuePM2-R1	Ozawa International Symposium (3rd Intl. Symp. on Oxidative Stress for Sustainable Development of Human Beings)
Tue. 28 Nov. 2023 / Room: Dreams 1
Session Chairs: Fuhua Yang; Christian Andre Amatore; Session Monitor: TBA

17:15: [OxidativeTuePM212] OL
PROTECTION OF VOCAL FOLD BY BASIC FIBROBLAST GROWTH FACTOR
Shigeru Hirano¹ ; ¹Kyoto Prefectural U. of Medicine, Kyoto, Japan;
Paper Id: 129 [Abstract]

Background: The vocal fold is a vibratory mucosa essential for creating voice, but it is fragile after injury, and once it is scarred, voice becomes hoarse and harsh, which is difficult to recover. Our previous research indicated that oxidative stress can cause scarring of injured vocal folds, and anti-oxidant can avoid the scarring. Basic fibroblast growth factor (FGF) has been proved to have anti-oxidant effects in vascular sclerosis. The current study examined the effects of FGF for preservation of the vocal fold after injury. Materials and Methods: Sprague Dawley rats were used as the model of vocal fold injury. Unilateral vocal fold was resected and immediately treated with FGF or saline injection (sham control). Scarring of the vocal folds were assessed at 2 months after the injury. Results: FGF group showed better wound healing with less scarring as compared to the sham. Hyaluronic acid was significantly better preserved. Gene expression analyses indicated significantly higher expression of HAS and HGF in FGF group. Conclusion: FGF was revealed to prevent vocal fold scarring through anti-fibrotic and possibly anti-oxidant effects.