ORALS

SESSION: OxidativeWedPM1-R1	Yoshikawa International Symposium (2nd Intl. Symp. on Oxidative Stress for Sustainable Development of Human Beings)
Wed. 30 Nov. 2022 / Room: Ballroom B
Session Chairs: Koji Fukui; Koji Abe; Session Monitor: TBA

14:00: [OxidativeWedPM105] OS Plenary
Twendee X improves cognitive function in vitamin E-deficient mice
Koji Fukui¹ ; Fuhua Yang² ; Yugo Kato¹ ; Yoshiaki Harakawa³ ; Toshikazu Yoshikawa⁴ ; Haruhiko Inufusa⁵ ; ¹Shibaura Institute of Technology, Saitama, Japan; ²Division of Anti-Oxidant Research, Gifu University, Gifu, Japan; ³Division of Anti-oxidant Research, Gifu University, Gifu, Japan; ⁴Louis Pasteur Medical Research Center, Kyoto, Japan; ⁵TIMA, Gifu, Japan;
Paper Id: 419 [Abstract]

Reactive oxygen species may attack several living organs and gradually accumulate oxidative products such as lipid hydroperoxides. Recently, several kinds of evidence have been demonstrated that accumulation of oxidative products induces onset and progression of several severe diseases such as inflammations, cardiovascular events and more [1]. One severe disease is dementia, and it also deeply relates to senescence. To prevent age-related neurodegenerative disorders, treatment with antioxidant is effective. Twendee X, a mixed antioxidant supplement contains 8 substances [2] and anyone can buy it. However, the detailed scientific evidence is not enough. In this study, we fed Twendee X to vitamin E-deficient mice and check cognitive function.<br />We created vitamin E-deficient mice and Twendee X treated for one month. Cognitive function was measured by the Morris Water maze task [3].<br />Vitamin E deficient mice were significantly decrease learning ability. Treatment with Twendee X was significantly improved it. Brain neurotrophic factors in Twendee X-treated mouse were significantly increase compared to the untreated groups.<br />Twendee X has a potential as a powerful antioxidant supplement through protection against neuronal dysfunction.

References:
[1] Yoshida, N, Kato, Y, Takatsu, H, et al., Biomedicines 10 (2022) 28.\n[2] Tadokoro, K, Morihara, R, Ohta, Y, et al., J Alzheimer’s Dis, 71 (2019) 1063-1069.\n[3] Fukui, K, Onodera, K, Shinkai, T, et al., Ann NY Acad Sci, 928 (2001) 169-176.

SESSION: OxidativeWedPM2-R1	Yoshikawa International Symposium (2nd Intl. Symp. on Oxidative Stress for Sustainable Development of Human Beings)
Wed. 30 Nov. 2022 / Room: Ballroom B
Session Chairs: Hironori Nakagami; Session Monitor: TBA

16:45: [OxidativeWedPM211] OS
The effect of anti-oxidant Twendee X on swallowing function in an animal model of dysphagia in guinea pigs
Yoichiro Sugiyama¹ ; Keiko Hashimoto² ; Shigeru Hirano³ ; Haruhiko Inufusa⁴ ; Toshikazu Yoshikawa⁵ ; ¹Department of Otolarynogolgy-Head and Neck Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan; ²Department of Otolaryngology-Head and Neck Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan; ³Kyoto Prefectural University of Medicine, Kyoto, Japan; ⁴TIMA, Gifu, Japan; ⁵Louis Pasteur Medical Research Center, Kyoto, Japan;
Paper Id: 433 [Abstract]

Dysphagia is one of the significant concerns in the aging society. In particular, the elderly with swallowing impairment caused by either central or peripheral nervous systems has difficulty in oral intake, possibly resulting in fatal pneumonia. As such, the development of a therapeutic approach that decreases in the risk of aspiration in dysphagic patients remains a pressing issue for medical doctors. The purpose of our study was to establish the animal model of dysphagia and test the hypothesis that decreased pharyngeal constriction caused by the denervation of the vagal efferent nerves can be improved by the application of the anti-oxidant agent. Thus, we developed the animal model in guinea pigs in which the pharyngeal branch of the vagus nerve was cut and initially evaluated swallowing function using videofluoroscopy. We then tested the effect of oral application of the anti-oxidant agent Twendee X on swallowing function in the dysphagia model animals. Insufficient pharyngeal constriction and pharyngeal residue were found in the denervated animals. The swallowing dysfunction was less pronounced in dysphagic animals with the administration of Twendee X. In conclusion, Twendee X could have a possible role in the improvement of swallowing function in dysphagic patients with decreased swallowing muscle strength caused by neuromuscular atrophy.