2024 - Sustainable Industrial Processing Summit
SIPS 2024 Volume 16. Intl. Symp on Electrochemistry, Molten Salts, Corrosion, Recycling and Battery

Editors:F. Kongoli, C.A. Amatore, R. Fehrmann, G. Kipouros, I. Paspaliaris, G. Saevarsdottir, R. Singh, R. Gupta, M. Halama, D. Macdonald, F. Wang, M. Barinova, F. Ahmed, C. Gaidau, X. Guo, K. Kolomaznik, H. Ozgunay, K. Tang, N.N. Thanh, S. Yefremova, K. Aifantis, Z. Bakenov, C. Capiglia, V. Kumar, A. U. H. Qurashi, A. Tressaud, R. Yazami
Publisher:Flogen Star OUTREACH
Publication Year:2024
Pages:243 pages
ISBN:978-1-998384-34-1 (CD)
ISSN:2291-1227 (Metals and Materials Processing in a Clean Environment Series)
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    A NOVEL STRATEGY FOR COLLAGEN EXTRACTION FROM TANNERY WASTES

    Qijue Chen1; Zhuoer Jiang1; Keyong Tang1;
    1ZHENGZHOU UNIVERSITY, Zhengzhou, China;
    Type of Paper: Regular
    Id Paper: 180
    Topic: 7

    Abstract:

    Plant-based enzymatic extraction of collagen from tannery rawhide trimmings can play an important role in the utilization of tannery solid waste. This study employed ficin enzyme derived from fig leaves waste for collagen extraction and compared the procedure with conventional acetic acid extraction. Response surface methodology (RSM) analysis revealed that ficin enzyme-soluble collagen (FSC) yielded 15.28% at a hydrolysis time of 39.27h, a ficin enzyme dose of 5.54%, and a mixing ratio of 15.87. In contrast, a lower yield of 9.27% was observed in the case of acetic acid-soluble collagen (ASC) at a hydrolysis time of 46.44h, a acidic acid concentration of 1.30mol/L, and a mixing ratio of 19.41. Fourier transform infrared spectroscopy (FTIR) confirmed the successful extraction of both ASC and FSC. Thermogravimetric analysis (TGA) indicated the higher thermal stability of ASC compared to FSC. The SEM images showed the presence of an organized porous-like structure in ASC and a separated irregular sheet-like mesh in FSC. After sonication treatment, there denoted the presence of a disorganized porous-like structure in UASC and a gathered irregular sheet-like mesh linked to each other in UFSC. Based on the negative ellipticity values, it can conclusion that PSC showed less integrity of triple helical structure while FSC exhibited well integrity of triple helical structure that comparable of standard collagen samples. Moreover, the present research confirmed that an ultrasound treatment at 140W for 30 min did not significantly disturb the triple helical structure of collagen. Notably, FSC and UFSC, with comparable UV absorption, seemed to be less proteinaceous impurity than other collagens with different extraction conditions. Hence, the results confirmed that appropriate sonication treatment would not cause contamination of non-collagenous proteins.

    Keywords:

    Tannery wastes; collagen extraction; ficin enzyme

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    Cite this article as:

    Chen Q, Jiang Z, Tang K. (2024). A NOVEL STRATEGY FOR COLLAGEN EXTRACTION FROM TANNERY WASTES. In F. Kongoli, C.A. Amatore, R. Fehrmann, G. Kipouros, I. Paspaliaris, G. Saevarsdottir, R. Singh, R. Gupta, M. Halama, D. Macdonald, F. Wang, M. Barinova, F. Ahmed, C. Gaidau, X. Guo, K. Kolomaznik, H. Ozgunay, K. Tang, N.N. Thanh, S. Yefremova, K. Aifantis, Z. Bakenov, C. Capiglia, V. Kumar, A. U. H. Qurashi, A. Tressaud, R. Yazami (Eds.), Sustainable Industrial Processing Summit Volume 16 Intl. Symp on Electrochemistry, Molten Salts, Corrosion, Recycling and Battery (pp. 178-189). Montreal, Canada: FLOGEN Star Outreach