| [Solid and liquid wastes from industrial processes: Innovations in material recovery and environmental protection] Utilization of Protein By-Products in 3D Bioprinting Utilization of Protein By-Products in 3D Bioprinting Eva Achbergerova1; Lenka Musilova2; Lenka Vitkova2; Ales Mracek2; Jiri Pecha1; 1TOMAS BATA UNIVERSITY IN ZLIN, FACULTY OF APPLIED INFORMATICS, CEBIA-TECH, Zlin, Czech Republic; 2TOMAS BATA UNIVERSITY IN ZLIN, Zlin, Czech Republic; PAPER: 102/Recycling/Keynote (Oral) SCHEDULED: 16:45/Mon. 28 Nov. 2022/Arcadia 2 ABSTRACT: Three-dimensional (3D) bioprinting has been employed in recent years as an attractive method for tissue engineering. [1] Using a 3D bioprinter allows proper distribution and positioning of biomaterials and cells to create desired constructs. [2] One of the major challenges associated with 3D bioprinting is the development of materials that can be used as suitable bioink. Namely, hydrogels formed by biopolymers (collagen, gelatine, hyaluronan, etc.) are of particular interest because of their capability to mimic the cell’s extracellular matrix (ECM). [3] The present work is aimed at the preparation and direct embedding of cells in 3D printed materials. Although native collagen is the major structural component of ECM, this biopolymer is less applicable for hydrogel preparation suitable for microextrusion due to its lower viscosity as well as accessibility to chemical modification. [4,5] Therefore, bovine or rabbit gelatines prepared from industrial collagen by-products (waste) were used in this study. Moreover, gelatines were mixed with hyaluronan and chemically cross-linked by glutaraldehyde to acquire materials with appropriate properties for 3D bioprinting. During the cross-linking, forming hydrogels were mixed with fluorescently stained fibroblasts and printed. Finally, cell distribution within the printed material was investigated using fluorescent imaging. In conclusion, chemically cross-linked hydrogels composed of biopolymers were prepared as a potentially promising bioink with application in 3D printing. References: [1] Blaeser, A., Campos, D. F. D., & Fischer, H. Current Opinion in Biomedical Engineering 2 (2017) 58-66 [2] Matai, I., Kaur, G., Seyedsalehi, A., McClinton, A., Laurencin, C. T. Biomaterials 226 (2020) 119536. [3] Van Vlierberghe, S., Dubruel, P., & Schacht, E. Biomacromolecules, 12 (2011) 1387-1408. [4] Mazzocchi, A., Devarasetty, M., Huntwork, R., Soker, S., & Skardal, A. Biofabrication, 11(1) (2019) 015003-15014. [5] Spicer, C. D. Polymer Chemistry 11 (3) (2020) 184-219. |
