2022-Sustainable Industrial Processing Summit
SIPS2022 Volume 18 Intl. Symp on Advanced Materials, Polymers, Composite, Nanomaterials, Nanotechnologies and Manufacturing
| Editors: | F. Kongoli, F. Marquis, N. Chikhradze, T. Prikhna, M. De Campos, S. Lewis, S. Miller, S. Thomas. |
| Publisher: | Flogen Star OUTREACH |
| Publication Year: | 2022 |
| Pages: | 290 pages |
| ISBN: | 978-1-989820-68-1(CD) |
| ISSN: | 2291-1227 (Metals and Materials Processing in a Clean Environment Series) |
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Development of Biocompatible and Fluorescent Gelatin Nanoparticles for Cells Labeling
Ying Pei1; Ying Pei1;1ZHENGZHOU UNIVERSITY, Zhengzhou, China;
Type of Paper: Regular
Id Paper: 152
Topic: 62
Abstract:
Cell imaging carriers with good biocompatibility have aroused wide attention.[1] Carbon quantum dots (CQDs) have attracted a great deal of attention due to their excellent properties, which is need to capsulated with non-toxic materials because of its biological toxicity.[2, 3] Gelatin has been widely used as a delivery vehicle on account of its good biocompatibility and biodegradability.[4] In this work, fluorescent gelatin nanoparticles (GNPs) were successfully fabricated by simple-cocervation and UV-crosslinking method with carbon quantum dots (CQDs) and fluorescein isothiocyanate (FITC) as fluorescence factors. The morphology and size were characterized by TEM and particle size analyzer. The average diameter of the gelatin nanoparticles (GNPs) is estimated at 390±50 nm. Meantime, the CQDs/GNPs have fluorescent properties with maximum emission at 416nm, with a slight 6±1nm blue-shift compared with CQDs. In vitro cytotoxicity test suggested that CQDs/GNPs and FITC/GNPs had not obvious toxic effect on L929 cells compared to that of individual CQDs and FITC. By confocal microscope observation, CQDs/GNPs and FITC/GNPs could bind to L929 cells for labeling. The results showed that gelatin nanoparticles have excellent fluorescence luminescence performance, including gelatin particles could be an ideal carriers for fluorescence factors. This work provided a new pathway for fabricating gelatin-based carriers for cell labeling and imaging.
Keywords:
Biodegradable; Biomass;References:
Reference:1. Chen, W., et al., Synthesis of graphene quantum dots from natural polymer starch for cell imaging. Green Chem, 2018. 20(19): p. 4438-4442.
2. Liu, J.H., et al., Carbon "Quantum" Dots for Fluorescence Labeling of Cells. ACS Appl Mater Interfaces, 2015. 7(34): p. 19439-45.
3. Molaei, M.J., A review on nanostructured carbon quantum dots and their applications in biotechnology, sensors, and chemiluminescence. Talanta, 2019. 196: p. 456-478.
4. Nezhad-Mokhtari,P., et al., Development of biocompatible fluorescent gelatin nanocarriers for cell imaging and anticancer drug targeting. J Mater Sci, 2018. 53(15): p. 10679-10691.