2022-Sustainable Industrial Processing Summit
SIPS2022 Volume 15 Yoshikawa Intl. Symp Oxidative Stress of Human Beings
| Editors: | F. Kongoli, H. Inufusa, C. Amatore, H. Chen, W. Huang, H. Goor. |
| Publisher: | Flogen Star OUTREACH |
| Publication Year: | 2022 |
| Pages: | 152 pages |
| ISBN: | 978-1-989820-62-9(CD) |
| ISSN: | 2291-1227 (Metals and Materials Processing in a Clean Environment Series) |
CD shopping page
Nanoelectrodes for Intracellular Measurements of Reactive Oxygen and Nitrogen Species in Single Living Cells
Christian Andre Amatore1; Keke Hu2; Xiaoke Yang3; Xin-Wei Zhang4; Yan-Ling Liu3; Alexander Oleinick5; Irina Svir6; Michael V Mirkin7; Wei-Hua Huang3;1CNRS & PSL, FRENCH ACADEMY OF SCIENCES, Paris, France; 2GOTEBORG UNIVERSITY, Gothenburg, Sweden; 3WUHAN UNIVERSITY, Wuhan, China; 4KEY LABORATORY OF ANALYTICAL CHEMISTRY FOR BIOLOGY AND MEDICINE, COLLEGE OF CHEMISTRY AND MOLECULAR SCIENCES, Wuhan, China; 5CNRS, Paris, France; 6ECOLE NORMALE SUPERIEURE, DEPARTMENT CHEMISTRY, PARIS, France; 7DEPARTMENT OF CHEMISTRY AND BIOCHEMISTRY, QUEENS COLLEGE-CUNY, Flushing, United States;
Type of Paper: Regular
Id Paper: 112
Topic: 54
Abstract:
Reactive oxygen and nitrogen species (ROS and RNS) play important roles in various physiological processes (e.g., phagocytosis) and pathological conditions (e.g., cancer) during episodes involving Oxidative Stress conditions. The primary ROS/RNS, viz., hydrogen peroxide, peroxynitrite ion, nitric oxide, and nitrite ion, can be oxidized at different electrode potentials and therefore detected and quantified by electroanalytical techniques. Nanometer-sized electrochemical probes are especially suitable for measuring ROS/RNS in single cells and cellular organelles. In this paper, we will survey recent advances in localized measurements of ROS/RNS inside single cells and discuss several methodological issues, including optimization of nanoelectrode geometry, precise positioning of an electrochemical probe inside a cell, and interpretation of electroanalytical data.
Application of this method will be presented for detection of ROS/RNS in human cancer cells and for demonstrating the existence of homeostatic control in phagolysosomes during phagocytosis by macrophages.
We will also show using these methods that remediation of Oxidative Stress in neurons artificially placed under Parkinson Disease conditions avoids the impeachment of synaptic communication when the neurons are pre-treated with Harpagide, a natural sugar derivative which alleviate the oxide stress borne by mitochondrions.
Keywords:
Anti-Oxidants; Mitochondria; Cancer Cells Proliferation; Macrophages; Nitric Oxide; Oxidative Stress; Peroxynitrite; Phagocytosis; Reactive Nitrogen Species (RNS); Reactive Oxygen Species (ROS);References:
1. See for example F. Murad, in Nobel Lecture for Physiology or Medicine, 1998,2. C Amatore, S. Arbault, M. Guille, F. Lemaître: Electrochemical monitoring of single cell secretion: vesicular exocytosis and oxidative stress. Chem. Rev. 108 (2008) 2585–2621.
3. C. Amatore, S. Arbault, D. Bruce, P. de Oliveira, M. Erard, M. Vuillaume: Analysis of individual biochemical events based on artificial synapses using ultramicroelectrodes: Cellular oxidative burst. Faraday Discuss. 116 (2000) 319-303.
4. Y. Wang, J. Noël, J. Velmurugan, W. Nogala, M.V. Mirkin, C. Lu, M. G. Collignon, F. Lemaitre, C. Amatore: Nanoelectrodes for determination of reactive oxygen and nitrogen species inside murine macrophages. Proc. Natl. Acad. Sci. U. S. A. 109 (2012) 11534-11539.
5. Y. Li, K. Hu, Y. Yu, S.A. Rotenberg, C. Amatore, M.V. Mirkin: Direct electrochemical measurements of reactive oxygen and nitrogen species in nontransformed and metastatic human breast cells. J. Am. Chem. Soc. 139 (2017) 13055-13062 (see corrected address in J. Am. Chem. Soc. 140 (2018) 3170−3170).
6. X.W. Zhang, Q.F. Qiu, H. Jiang, F.L. Zhang, Y.L. Liu, C. Amatore, W.H Huang: Real-Time intracellular measurements of ROS and RNS in living cells with single core-shell nanowire electrodes. Angew. Chem. Int. Ed. 56 (2017) 12997-13000.
7. X.W. Zhang, A. Oleinick, H. Jiang, Q.L. Liao, Q.F. Qiu, I. Svir, Y.L. Liu, C. Amatore, W.H. Huang: Electrochemical monitoring of ROS/RNS homeostasis within individual phagolysosomes inside single macrophages. Angew. Chem. Int. Ed. 58 (2019) 7753-7756.
8. R.R. Pan, K. Hu, D. Jiang, U. Samuni, M.V. Mirkin: Electrochemical resistive-pulse sensing. J. Am. Chem. Soc. 141 (2019) 19555-19559.
9. Y. Tang, X.K. Yang, X.W. Zhang, W.T. Wu, F.L. Zhang, H. Jiang, Y.L. Liu, C. Amatore, W.H. Huang: Harpagide, a natural product, promotes synaptic vesicle release as measured by nanoelectrode amperometry. Chem. Sci., 11, 2020, 778-785.