| Effect of antioxidant Twendee X on radiation-induced oxidative stress Yoshiaki Harakawa1; Fuhua Yang1; Toshikazu Yoshikawa2; Haruhiko Inufusa3; 1DIVISION OF ANTI-OXIDANT RESEARCH, GIFU UNIVERSITY, Gifu, Japan; 2LOUIS PASTEUR CENTER, Kyoto, Japan; 3TIMA, Gifu, Japan; PAPER: 418/Oxidative/Regular (Oral) SCHEDULED: 15:15/Mon. 28 Nov. 2022/Ballroom B ABSTRACT: It is known that when a living organism is exposed to radiation, water molecules in the body ionize, producing reactive oxygen species, which in turn cause inflammation.<br />Twendee X (TwX) is an antioxidant supplement consisting of eight ingredients (coenzyme Q10, vitamin C, vitamin B2, niacin, L-cystine, succinic acid, fumaric acid, and L-glutamine). We investigated whether TwX and other substances can inhibit oxidative stress generated by radiation.<br />C57BL/6J mice were orally administered the test substance TwX (15 mg/kg) for 3 days at 24-hour intervals, irradiated with 6 Gy of radiation on the fourth day, and blood samples were taken 72 hours later, and survival was observed as is. Vitamin C (15 and 150 mg/kg), Resveratrol, Astaxanthin, Protandim, and Pycnogenol were used as antioxidants for comparison to TwX. Plasma hydroperoxide level was measured as blood oxidative stress (d-ROMs test).<br />Increased d-ROMs test value by radiation was reduced by one third in the group treated with TwX (15 mg/kg) before irradiation for 3 days (t-test, p<0.05), and the oxidative stress level was lower in the group treated for 7 days than in normal mice. In addition, the administration of TWX suppressed radiation-induced deaths.<br />Vitamin C (150 mg/kg) and Astaxanthin treatment groups reduced d-ROMs test values, but the difference was not statistically significant. The low concentrations of vitamin C (15 mg/kg), Pycnogenol, and Protandim did not change the d-ROMs test values compared to the control group, and for the Resveratrol group, the d-ROMs test values increased compared to the control group. Furthermore, Twendee Mtcontrol (TwM), which added seven active ingredients to TwX, reduced radiation-related deaths with fewer doses than TwX.<br />TwX significantly reduced radiation-induced oxidative stress at a small dose of 15 mg/kg, while other single antioxidants with normal amount have no significant improvement in animal radiation model. High dose antioxidants may cause side effects. TwX, TwM are composed only of substances that exist in the human body, and a small dose of them showed high antioxidant activity. This may have reduced inflammation caused by oxidative stress, ultimately leading to an increase in survival rate. The antioxidant potential of Twendee series in this experiment suggests the possibility of future applications for various oxidative stress-related diseases. References: [1] Tadokoro K, et al. Clinical Benefits of Antioxidative Supplement Twendee X for Mild Cognitive Impairment: A Multicenter, Randomized, Double-Blind, and Placebo-Controlled Prospective Interventional Study. J Alzheimers Dis. 2019;71(3):1063-1069.<br />[2] Yamamoto T, Kinoshita M, Shinomiya N, Hiroi S, Sugasawa H, Matsushita Y, Majima T, Saitoh D, Seki S. Pretreatment with ascorbic acid prevents lethal gastrointestinal syndrome in mice receiving a massive amount of radiation. J. Radiat. Res., 2010. 51, 145-156．<br />[3] Yoshikawa T, et al. [All about Free Radicals – from Health to Environmental Pollution -] Kasseisanso , Free Radical no subete – Kennkou kara kannkyouosen made – (in Japanese), Maruzen Publishing, 2000, 78-79.<br />[4] Seki Y. [Evaluation of total oxidative stress by d-ROMs testing] d-ROMs test niyoru sankasutoresu sougouhyouka (in Japanese). Seibutsu siryou bunseki (Journal of analytical bio-science), 2010. 32(4), 301-306. |
