SIPS 2018

DETAILLED PROGRAM OVERVIEW

Maintenance of the Cell Motility in the Tunic of Halocynthia Roretzi
Yoko Kato¹;
¹TOHOKU GAKUIN UNIVERSITY, Tagajo, Japan;
PAPER: 273/AdvancedMaterials/Regular (Oral)
SCHEDULED: 12:35/Wed./Guaratiba (60/2nd)

ABSTRACT:
The previous reports have indicated that the tunic of Halocynthia roretzi, which has chitin sulfate-like polysaccharide and cellulose Ibeta, responds to various stimulus, including mechanical stimuli and neurotransmitters, and deforms as a result [1 -4]. Moreover, the water content of the tunic was changed when the deformation occurred [1]. While the tunic in the artificial seawater increased its mass at 5 °C, whose temperature reduced the activity of the metallo-protease secreted from the cells in the tunic, the propagation of bacteria in the tunic would be prevented [1]. However, whether or not the condition of the cells in the tunic could be maintained at 5 °C has been barely examined. If the activity of the cells in the tunic is maintained in the artificial water, the conditions of the tunic could be controlled on its own. In this study, the motility of the cell obtained from the tunic, kept at 5 °C for 10 days, by centrifugation (1000G, 7 min) was examined. In addition, the contents of the dissolved organic matter and nitrate in the supernatant and seawater used for keeping the tunic were evaluated by the absorbance at 250 -350 nm and 220 nm in the spectroscopic analysis, respectively. The actively moving cells observed although the tunic were just kept in the artificial seawater. In the meantime, the contents of the dissolved organic matter and nitrate in the supernatant were more than those in the artificial seawater used for keeping the tunic. When these two types of the solutions were kept for 10 days at 5 °C, most of the samples indicated the decrease in the contents of the dissolved organic matter and nitrate. The tunic of Halocynthia roretzi could maintain its condition suitable for the cells independently.

References:
[1] Y. Kato, Journal of Biomechanical Science and Engineering, Mar. 2010; 5(2): 163-174. <br />[2] Y. Kato, Proceedings of ISOPE-2011, Maui.<br />[3] Y. Kato, Proceedings of ASME 2012 International Mechanical Engineering Congress and Exposition, Nov. 2012: 607-613.<br />[4] Y. Kato, Open Chemistry Journal, 2018; 5: 1-17.