2018 - Sustainable Industrial Processing Summit & Exhibition
4-7 November 2018, Rio Othon Palace, Rio De Janeiro, Brazil
Seven Nobel Laureates have already confirmed their attendance: Prof. Dan Shechtman, Prof. Sir Fraser Stoddart, Prof. Andre Geim, Prof. Thomas Steitz, Prof. Ada Yonath, Prof. Kurt Wüthrich and Prof. Ferid Murad. More than 400 Abstracts Submitted from about 60 Countries.
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    Highly Sensitive Electrochemical Bioanalysis of Unicellular Samples based on Au@Pt Nanaostructures Modified Diamond Microelectrode Arrays
    Xuemei Wang1; Hui Jiang1; Alberto Pasquarelli2; Chunmei Li1; Hang Zhang1; Jun Ruan1;
    1SOUTHEAST UNIVERSITY, Nanjing, China; 2ULM UNIVERSITY, Ulm, Germany;
    PAPER: 229/Electrochemistry/Regular (Oral)
    SCHEDULED: 15:15/Tue./Copacabana B (150/1st)



    ABSTRACT:
    In this work, aiming at the construction of a sensitive, disposable system for the bioanalysis of unicellular samples, we report a single cell detection electrochemical (SCDE) platform based on Au@Pt nanostructures modified boron-doped nanocrystalline diamond microelectrode arrays (BNCD-MEAs) with 16 channels. The SCDE platform could be readily adapted for a highly sensitive detection of H<sub>2</sub>O<sub>2</sub> released from stimulated single cell by ascorbic acid (AA), which could be utilized to distinguish cancer unicellular and normal unicellular ones. The Au@Pt nanostructures have good electrocatalytic activity for the H<sub>2</sub>O<sub>2</sub> detection and were modified in the BNCD-MEAs by Pt NPs which were electrodeposited onto the BNCD-MEAs through an electrodeposition approach. As a result, this SCDE platform exhibited a wide linear range with a low detection for H<sub>2</sub>O<sub>2</sub> determination. Furthermore, it also displayed satisfactory selectivity, excellent stability, and good reproducibility. The developed method opens a new pathway to clinical bioassay.

    References:
    [1] Du, T.; ; Zhao, C.; ur Rehman, F.; Lai, L.; Li, X.; Sun, Y.; Luo, S.; Jiang, H.; Gu, N.; Selke, M.; Wang, X., In Situ Multimodality Imaging of Cancerous Cells Based on a Selective Performance of Fe2+-Adsorbed Zeolitic Imidazolate Framework-8. Advanced Functional Materials 2017, 27 (5), 1603926;
    [2] Nascimento, R. A.; Ozel, R. E.; Mak, W. H.; Mulato, M.; Singaram, B.; Pourmand, N., Single Cell "Glucose Nanosensor" Verifies Elevated Glucose Levels in Individual Cancer Cells. Nano letters 2016, 16 (2), 1194-200.
    [3] Lai, L.; Jiang, X.; Han, S.; Zhao, C.; Du, T.; Rehman, F. U.; Zheng, Y.; Li, X.; Liu, X.; Jiang, H.; Wang, X., In Vivo Biosynthesized Zinc and Iron Oxide Nanoclusters for High Spatiotemporal Dual-Modality Bioimaging of Alzheimer's Disease. Langmuir : the ACS journal of surfaces and colloids 2017, 33 (36), 9018-9024.
    [4] Actis, P.; Maalouf, M. M.; Kim, H. J.; Lohith, A.; Vilozny, B.; Seger, R. A.; Pourmand, N., Compartmental genomics in living cells revealed by single-cell nanobiopsy. ACS nano 2014, 8 (1), 546-53.
    [5] Adam Seger, R.; Actis, P.; Penfold, C.; Maalouf, M.; Vilozny, B.; Pourmand, N., Voltage controlled nano-injection system for single-cell surgery. Nanoscale 2012, 4 (19), 5843-6.
    [6] Zhang, X.; Ding, S. N., Graphite paper-based bipolar electrode electrochemiluminescence sensing platform. Biosensors & bioelectronics 2017, 94, 47-55.
    [7] Shen, R.; Liu, P.; Zhang, Y.; Yu, Z.; Chen, X.; Zhou, L.; Nie, B.; Zaczek, A.; Chen, J.; Liu, J., Sensitive Detection of Single-Cell Secreted H2O2 by Integrating a Microfluidic Droplet Sensor and Au Nanoclusters. Analytical chemistry 2018, 90 (7), 4478-4484.
    [8] Kniss-James, A. S.; Rivet, C. A.; Chingozha, L.; Lu, H.; Kemp, M. L., Single-cell resolution of intracellular T cell Ca(2+) dynamics in [9] Gao, Y.; Xu, W.; Mason, B.; Oakes, K. D.; Zhang, X., Anion-exchange membrane-separated electrochemical cells enable the use of sacrificial anodes for hydrogen peroxide detection with enhanced dynamic ranges. Electrochimica Acta 2017, 246, 707-711.
    [10] Ruan, J.; Zhang, W.; Zhang, H.; Chen, Y.; Rehman, F. U.; Jiang, H.; Strehle, S.; Pasquarelli, A.; Wang, X., Highly sensitive electrochemical detection of living cells based on diamond microelectrode arrays. Chinese Chemical Letters 2017.