SIPS 2018

DETAILLED PROGRAM OVERVIEW

Microbiological Quality of Bivalves and Their Water Beds Along the Mediterranean Coast of Egypt
Moustafa El Shenawy¹;
¹NATIONAL RESEARCH CENTER, Cairo, Egypt;
PAPER: 20/Environmental/Regular (Oral)
SCHEDULED: 15:55/Wed./Pontal (50/2nd)

ABSTRACT:
There is widespread concern about the safety of shellfish, which are harvested from quite shallow locations along the Mediterranean coast of Egypt. Investigation of some microbiological properties of the collected bivalves and their water beds, and surface and bottom areas, were done during four seasonal sampling cruises from March, 2016 to February, 2017 at eleven stations. All samples were analyzed for the total plate count, fecal pollution indicators, bacteria (total coliforms, E.coli, and fecal streptococci), and the prevalence of three emerging food-borne pathogens, including Listeria monocytogenes, Campylobacter, and E.coli 0157:H7. In addition, some environmental parameters of the coastal water samples, including temperature, salinity, dissolved oxygen, and pH were also measured. Sampling techniques were done according to IOS standers, and the membrane filtration technique was applied using the total plate count agar and the respective selective media. Identification using the biochemical tests was done and the final counts were calculated as cfu/100ml/gm water/meat. Fecal pollution bacterial counts ranged from <1 to 104 of all the detected microorganisms, depending on the investigated area and dates of sampling. The incidence of Listeria monocytogenes in the examined water samples varied widely from 0 to 15%. Campylobacter was found in percentages of 2 to 20%. E.coli 0157:H7 was detected in 3 to 25% of the investigated locations. There was an association between the fecal contamination indicators and the presence of the studied pathogens. The same trend was observed in the oyster meat samples. These results may help to develop sanitary strategy/strategies for better Mollusca shellfish safety.

References:
[1] Pierce, Melissa L. ; Ward, J. Evan ; Holohan, Bridget A. ; Zhao, Xiaowei ; Hicks, Randall E./ The influence of site and season on the gut and pallial fluid microbial communities of the eastern oyster, Crassostrea virginica (Bivalvia, Ostreidae) : community-level physiological profiling and genetic structure. In: Hydrobiologia. 2016 ; Vol. 765, No. 1. pp. 97-113
[2] Bayat, Zeynab; Hassanshahian, Mehdi; Hesni, Majid A./Enrichment and isolation of crude oil degrading bacteria from some mussels collected from the Persian Gulf. In: Marine Pollution Bulletin. 2015; Vol. 101, No. 1. pp. 85-91
[3] Lasa, A., Diéguez, A.L. & Romalde, J.L. Antonie van Leeuwenhoek (2014) 105: 335.