Editors: | Kongoli F, Marquis F, Chikhradze N |
Publisher: | Flogen Star OUTREACH |
Publication Year: | 2017 |
Pages: | 590 pages |
ISBN: | 978-1-987820-69-0 |
ISSN: | 2291-1227 (Metals and Materials Processing in a Clean Environment Series) |
In this study, a potentiometric urea biosensor through the immobilization of urease enzyme onto CS / Magnesium (Mn3O4) nanocomposite has been fabricated on glass filter paper. A copper wire (diameter = 200 µm) is attached with nanoparticles to extract the voltage output signal. The shape and dimensions of Magnesium (Mn3O4) nanoparticles are investigated by scanning electron microscopy and the average diameter is approximately 70-90 nm. Structural quality of Magnesium (Mn3O4) nanoparticles is confirmed from x-ray powder diffraction measurements A physical adsorption method is adopted to immobilize the surface of CS/ Magnesium (Mn3O4) nano composite. The potentiometric sensitivity curve has been measured over the concentration range (1x 10-4 to 8 x 10-2 M) of urea electrolyte solution revealing that the fabricated biosensor holds good sensing ability with a linear slope curve of ~45mV/decade. In addition, the presented biosensor shows good reusability, selectivity, reproducibility and resistance against interferers along with the stable output response of ~8 seconds.