2014-Sustainable Industrial Processing Summit
SIPS 2014 Volume 5: Composite, Ceramic, Quasi-crystals, Nanomaterials & Coatings
| Editors: | Kongoli F |
| Publisher: | Flogen Star OUTREACH |
| Publication Year: | 2014 |
| Pages: | 578 pages |
| ISBN: | 978-1-987820-07-2 |
| ISSN: | 2291-1227 (Metals and Materials Processing in a Clean Environment Series) |
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L-α-diheptanoylphosphatidycholine (DHPC) Capped Gold Nanoparticles: Synthesis
Sourbh Thakur1;1UNIVERSITY OF JOHANNESBURG, Amritsar, India;
Type of Paper: Regular
Id Paper: 272
Topic: 14
Abstract:
L-α-diheptanoylphosphatidycholine (DHPC) capped gold (Au) nanoparticles (NPs) were synthesized to explore their application for future energy. The purpose of choosing this phospholipid is simply based upon the fact that L-α-diheptanoylphosphatidycholine (DHPC)is micelle forming phospholipid due to its readily soluble nature in pure water. Reactions were performed by precisely controlling the temperature and monitoring simultaneously UV-Visible and fluorescence spectrophotometer measurements. UV-visible spectra of colloidal Au solutions were taken by UV spectrophotometer in the wavelength range of 200-900 nm to determine the absorbance due to Surface Plasmon Resonance (SPR) of nanometallic Au. The formation of Au NP was monitored in the visible absorption range of 540 nm. The hydrophobicity of DHPC was determined with the help of pyrene fluorescence measurements by using Hitachi fluorescence spectrophotometer. The pyrene emission spectra were recorded employing an excitation wavelength of 334 nm. Emission intensities I1 and I3 were measured at the wavelengths corresponding to the first and third vibronic bands located at 373 and 384 nm. Since DHPC is not a florescence active molecule, therefore pyrene has been used as a florescence probe in order to detect the DHPC gold NP interactions. I have used seed growth (S-G) method to synthesize lipid-capped Au NP. It seems that the citrate solubilized gold NP provides desired electrostatic interaction with positively charged ammonium group of phospho-choline zwitterionic head group of DHPC monomers that formed the micelles. Such an association is expected to reduce the interhead group repulsions existing in the stern-layer of micelle thus providing it a greater stability with enhanced hydrophobicity. The present results clearly explain a strong association between DHPC micelle and gold NP as evaluated by pyrene fluorescence measurement. Phospholipid-NP conjugate materials are foreseen as the basic components for bionanometallic devices with potential applications in sensors, gene sequencing, and biocatalysis.